Eimeria maxima gametocyte antigens: potential use in a subunit maternal vaccine against coccidiosis in chickens

摘要: Affinity-purified gametocyte antigens (APGA) from Eimeria maxima, emulsified in Freund's adjuvant, were injected intramuscularly into breeding hens on two or three occasions. As a result, progeny of the immunized partially immune to infection with E. tenella and acervulina (with reduction total oocyst output 45-63% as compared untreated hens). Thus, APGA adjuvant appears have great potential part maternally applied vaccine against coccidiosis. The ability induce partial cross-species protection is most probably due existence conserved epitopes different species indicated by comparative Western blots maxima tenella. Surprisingly, itself also induced significant levels maternal immunity coccidiosis 12-35% progeny). In contrast purified alone, crude extracts gametocytes well other developmental stages, little if any despite provoking production large amounts parasite-specific IgG, including antibodies APGA. This result indicates that successful requires, addition good recognition protective antigens, exclusion irrelevant preparation.