An evaluation of styrene genotoxicity using several biomarkers in a 3-year follow-up study of hand-lamination workers.

摘要: Abstract A study employing several biomarkers of styrene exposure and genotoxicity was carried out in a group lamination (reinforced plastic) workers controls, who had been repeatedly sampled during 3-year period. Special attention will be paid to the last sampling (S.VI), reported here for first time. Styrene concentration breathing zone, monitored by personal dosimeters, urinary mandelic acid (MA) were measured as indicators external exposure. Blood samples assayed styrene-specific O 6 -guanine adducts DNA, N-terminal valine haemoglobin, DNA single-strand breaks (SSB), determined use single cell gel electrophoresis (Comet) assay), hypoxanthine guanine phosphoribosyl transferase (HPRT) mutant frequencies (MF) T-lymphocytes. -styrene adduct levels significantly higher exposed (5.9±4.9 adducts/10 8 dNp) compared laboratory controls (0.7±0.8 dNp; P =0.001). correlated with haemoglobin adducts, SSB parameters years employment. Styrene-induced detected (1.7±1.1 pmol/g globin), but not control (detection limit 0.1 globin). strongly indicators, HPRT MF. No significant correlation found parameters. statistically difference MF observed between laminators (22.3±10.6/10 ) (14.2±6.5/10 , =0.039). air, MA well employment age employees. ( =0.450) factory observed. Surprisingly, we differences sexes. When data from all measurements combined, women showed (geometric mean 15.4 vs. 11.2 men, =0.020). The styrene-exposed exhibited (tail moment (TM), tail length (TL) percentage (TP)) than adducts. or encompassing repeated same population over period are discussed respect mechanisms genotoxic effects interrelationship individual biomarkers.