Epidermal Growth Factor Receptor (EGFR)-mediated Positive Feedback of Protein-tyrosine Phosphatase ϵ (PTPϵ) on ERK1/2 and AKT Protein Pathways Is Required for Survival of Human Breast Cancer Cells
作者： Caroline E. Nunes-Xavier , Ari Elson , Rafael Pulido
关键词:
摘要: Increased tyrosine phosphorylation has been correlated with human cancer, including breast cancer. In general, the activation of kinases (TKs) can be antagonized by action protein-tyrosine phosphatases (PTPs). However, in some cases PTPs potentiate TKs. this study, we have investigated functional role PTPϵ cancer cell lines. We found up-regulation and receptor (RPTPϵ) MCF-7 cells MDA-MB-231 upon PMA, FGF, serum stimulation, which depended on EGFR ERK1/2 activity. Diminishing expression abolished AKT activation, decreased viability anchorage-independent growth cells. Conversely, stable lines expressing inducible high levels ectopic displayed higher growth. Our results demonstrate that is up-regulated activated lines, through EGFR, sustained pathway, generating a positive feedback regulatory loop required for survival
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