作者: Tom Payne , Colin Hanfrey , Amy L. Bishop , Anthony J. Michael , Simon V. Avery
DOI: 10.1016/J.FEBSLET.2008.01.009
关键词:
摘要: Accumulation of unfolded proteins in the endoplasmic reticulum (ER) causes stress and induces protein response (UPR). Genome-wide analysis translational regulation to UPR-inducing agent dithiothreitol Saccharomyces cerevisiae is reported. Microarray analysis, confirmed using qRT-PCR, identified transcript-specific regulation. Transcripts with functions ribosomal biogenesis assembly were translationally repressed. In contrast, mRNAs from known UPR genes, encoding transcription factor Hac1p, ER-oxidoreductase Ero1p ER-associated degradation (ERAD) Der1p, enriched polysomal fractions, indicating up-regulation. Splicing HAC1 mRNA shown be required for efficient loading.