Transcript-specific translational regulation in the unfolded protein response of Saccharomyces cerevisiae.

作者: Tom Payne , Colin Hanfrey , Amy L. Bishop , Anthony J. Michael , Simon V. Avery

DOI: 10.1016/J.FEBSLET.2008.01.009

关键词:

摘要: Accumulation of unfolded proteins in the endoplasmic reticulum (ER) causes stress and induces protein response (UPR). Genome-wide analysis translational regulation to UPR-inducing agent dithiothreitol Saccharomyces cerevisiae is reported. Microarray analysis, confirmed using qRT-PCR, identified transcript-specific regulation. Transcripts with functions ribosomal biogenesis assembly were translationally repressed. In contrast, mRNAs from known UPR genes, encoding transcription factor Hac1p, ER-oxidoreductase Ero1p ER-associated degradation (ERAD) Der1p, enriched polysomal fractions, indicating up-regulation. Splicing HAC1 mRNA shown be required for efficient loading.

参考文章(27)
John WB Hershey, Michael Mathews, Nahum Sonenberg, None, Translational control of gene expression Cold Spring Harbor Laboratory Press. ,(2000)
Rowan E. Chapman, Peter Walter, Translational attenuation mediated by an mRNA intron Current Biology. ,vol. 7, pp. 850- 859 ,(1997) , 10.1016/S0960-9822(06)00373-3
G. Johannes, M. S. Carter, M. B. Eisen, P. O. Brown, P. Sarnow, Identification of eukaryotic mRNAs that are translated at reduced cap binding complex eIF4F concentrations using a cDNA microarray Proceedings of the National Academy of Sciences of the United States of America. ,vol. 96, pp. 13118- 13123 ,(1999) , 10.1073/PNAS.96.23.13118
M. Inada, C. Guthrie, Identification of Lhp1p-associated RNAs by microarray analysis in Saccharomyces cerevisiae reveals association with coding and noncoding RNAs. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 101, pp. 434- 439 ,(2004) , 10.1073/PNAS.0307425100
Tetsushi Kawahara, Hideki Yanagi, Takashi Yura, Kazutoshi Mori, Endoplasmic Reticulum Stress-induced mRNA Splicing Permits Synthesis of Transcription Factor Hac1p/Ern4p That Activates the Unfolded Protein Response Molecular Biology of the Cell. ,vol. 8, pp. 1845- 1862 ,(1997) , 10.1091/MBC.8.10.1845
W. Zhou, G. M. Edelman, V. P. Mauro, Transcript leader regions of two Saccharomyces cerevisiae mRNAs contain internal ribosome entry sites that function in living cells Proceedings of the National Academy of Sciences of the United States of America. ,vol. 98, pp. 1531- 1536 ,(2001) , 10.1073/PNAS.98.4.1531
Thomas Preiss, Julie Baron-Benhamou, Wilhelm Ansorge, Matthias W Hentze, Homodirectional changes in transcriptome composition and mRNA translation induced by rapamycin and heat shock. Nature Structural & Molecular Biology. ,vol. 10, pp. 1039- 1047 ,(2003) , 10.1038/NSB1015