Genome-wide transcriptional analysis of Saccharomyces cerevisiae during industrial bioethanol fermentation.

摘要: Saccharomyces cerevisiae is widely applied in large-scale industrial bioethanol fermentation; however, little known about the molecular responses of yeast during fermentation processes. We investigated global transcriptional an strain S. continuous and fed-batch by oligonucleotide-based microarrays. About 28 62% all genes detected showed differential gene expression fermentation, respectively. The overrepresented functional categories differentially expressed overlapped with those fermentation. Downregulation glycosylation as well upregulation unfolded protein stress response was observed both processes, suggesting dramatic changes environment endoplasmic reticulum Genes related to ergosterol synthesis involved glycogen trehalose metabolism were downregulated Additionally, transcription carbohydrate coincided glucose limitation early main stage also found that late stage, cells exhibited similar but stronger process than process. Furthermore, repression has been suggested be a secondary model proposed explain Together, these findings provide insights into performance processes for production.