Potassium as a pluripotency-associated element identified through inorganic element profiling in human pluripotent stem cells.
作者： Victor J. T. Lin , Ashwini Zolekar , Yi Shi , Bhuvaneswari Koneru , Slobodan Dimitrijevich
DOI: 10.1038/S41598-017-05117-2
关键词:
摘要: Despite their well-known function in maintaining normal cell physiology, how inorganic elements are relevant to cellular pluripotency and differentiation human pluripotent stem cells (hPSCs) has yet be systematically explored. Using total reflection X-ray fluorescence (TXRF) spectrometry inductively coupled plasma mass (ICP-MS), we analyzed the components of with isogenic backgrounds distinct states pluripotency. The elemental profiles revealed that potassium content significantly differs when changes. Pharmacological treatment alters membrane permeability affected maintenance establishment via multiple mechanisms bona fide hPSCs reprogrammed cells. Collectively, report is a pluripotency-associated element provide novel insights into manipulation by regulating intracellular potassium.
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