An automated multi-flume actograph for the study of behavioral rhythms of burrowing organisms
作者： V. Sbragaglia , J. Aguzzi , J.A. García , D. Sarriá , S. Gomariz
DOI: 10.1016/J.JEMBE.2013.05.018
关键词:
摘要: Abstract In this study, we present and test the functioning of a automated multi-flume actograph that is able to simulate concomitant geophysical cycles (day-night hydrodynamic cycles) characterizing benthic environment continental margins. The burrowing Norway lobster ( Nephrops norvegicus , L.) was used device. system endowed with pumps pipe for periodical current flow generation. Monochromatic blue light cycle (472 nm) provided by submergible LED's lighting strips. Locomotor activity 8 individuals tracked 4 HD video cameras during 10 days trial. A customized video-imaging protocol in MATLAB calculated displacement animals (cm/min). tested simulating an Atlantic shelf scenario (i.e. intensity 4 · 10 − 3 μE/m 2 /s at 10 cm/s). Robust time series outputs nocturnal phase were reported, first laboratory evidence influence on burrow emergence species. Water increase inhibited movement generating dual reaction relation their phase. method presented here could be pivotal study unknown aspects ecology.
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