Structural features of mouse telomerase RNA are responsible for the lower activity of mouse telomerase versus human telomerase.
作者： Scott J. Garforth , Yan Yun Wu , Vinayaka R. Prasad
DOI: 10.1042/BJ20060456
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摘要: Human and mouse telomerases show a high degree of similarity in both the protein RNA components. telomerase is more active processive than telomerase. There are two key differences between hTR [human TR (telomerase RNA)] mTR (mouse TR) structures. First, contains only 2 nt upstream its template region, whereas human 45 nt. Secondly, region 5-nt alignment domain, that has We hypothesize these responsible for differential activities. Mutations were made mTR, changing length template, was reconstituted vitro using reverse transcriptase generated by translation. sequences with potential to form double-stranded helix (the P1 helix) as hTR, increase activity. The longer domain increases activity context helix. Thus contributes regulating level mammalian telomerases.
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