Sequence of the murine and human cellular myc oncogenes and two modes of myc transcription resulting from chromosome translocation in B lymphoid tumours.

摘要: The 15;12 chromosome translocation in murine plasmacytomas and the 8;14 human Burkitt lymphomas often link cellular myc oncogene to locus for constant regions of immunoglobulin heavy chains (CH locus). To clarify how why c-myc occurs, we have sequenced mouse genes correlated transcription with rearrangement. Both comprise three exons; second third encode polypeptide, which is conserved between mammals birds, particularly its more basic C-terminal half. Southern blots showed that four 12 lines linked near CH switch two joining region (JH) locus. Hence, recombination machinery may participate translocation, although common breakpoint around exon 1 does not resemble a region. Tumours breakpoints just 5' 1, or distant from c-myc, had normal mRNAs 2.25 2.4 kb, differ at their ends, while tumours within intron altered (2.1-2.7 kb lines), initiated 1. types probably yield same polypeptide. Since untranslocated allele was generally silent, must induce constitutive expression. presence mRNA immortal but non-tumorigenic lymphoblastoid cell implicate an immortalization step.