Twelve hour real-time PCR technique for the sensitive and specific detection of Salmonella in raw and ready-to-eat meat products

摘要: Rapid pathogen testing is vital to the food industry. Enzyme immunoassays (EIA) provide reliable negative results in 48 h, but a presumptive positive (suspect) EIA result must be confirmed by traditional culture methods, requiring an additional 72 h. Polymerase chain reaction (PCR) technology accepted as accurate diagnostic tool. However, PCR techniques can require several days. We sought develop rapid, real-time quantitative technique for detecting Salmonella spp. products. was inoculated into raw and ready-to-eat beef Total DNA extracted used template amplification LightCycler (Roche Diagnostics Corp., Idaho Technology Inc., Falls, ID) instrument. Salmonella-specific primers were designed amplify 251 base pair product from junction of SipB SipC. Fluorescently-labeled hybridization probes anneal detected down 1 colony forming unit/ml The correlated 100% those visual immunoprecipitate culture. methods using detect confirm presence or absence products within 12 h with increased sensitivity compared methods.