Effect of growth media on gene expression levels in Salmonella Typhimurium biofilm formed on stainless steel surface

摘要: Abstract Salmonella biofilm is recognized as a frequent source of cross-contamination and has been associated with food-borne outbreaks. It more meaningful for evaluating formation in food-based medium than laboratory-growth medium. In the present study, expression functional genes during Typhimurium grown meat-based standard growth substrates were determined by RT-qPCR assays. The number viable cells tested displayed specific behavior according to conditions. When was 7 days laboratory trypticase soy broth (TSB), increased, but when meat thawing loss (MTLB), gene inhibited compared corresponding planktonic cells. Relative MTLB lower that TSB. Positive relationships found between each TSB at 3, 5 incubation, their correlation did not changed variation media biofilm. A preliminary positive relationship also observed levels some genes. Our results suggested coordinately governed highly complex regulatory network, where this study ( adrA , bapA csgB csgD csrA rpoS invA sipB-C sdiA luxS ) played important roles.