Autophagy-Inducing Factor Atg1 Is Required for Virulence in the Pathogenic Fungus Candida glabrata.

摘要: Candida glabrata is one of the leading causes candidiasis and serious invasive infections in hosts with weakened immune systems. C. a haploid budding yeast that resides healthy hosts. Little known about mechanisms virulence. Autophagy ‘self-eating’ process developed eukaryotes to recycle molecules for adaptation various environments. speculated play role pathogen virulence by supplying sources essential proteins survival severe host Here, we investigated effects defective autophagy on was induced nitrogen starvation hydrogen peroxide (H2O2) glabrata. A mutant strain lacking CgAtg1, an autophagy-inducing factor, generated confirmed be deficient autophagy. The Cgatg1∆ sensitive H2O2, died rapidly water without any nutrients, showed high intracellular ROS levels compared wild-type CgATG1-reconstituted vitro. Upon infecting mouse peritoneal macrophages, higher mortality from phagocytosis macrophages. Finally, vivo experiments were performed using two models disseminated intra-abdominal candidiasis. significantly decreased CFUs organs models. These results suggest contributes conferring resistance unstable nutrient environments defense hosts, Atg1 novel fitness factor species.