Alkaline phosphatase levels and osteoprogenitor cell numbers suggest bone formation may contribute to peak bone density differences between two inbred strains of mice.
作者： H.P. Dimai , T.A. Linkhart , S.G. Linkhart , L.R. Donahue , W.G. Beamer
DOI: 10.1016/S8756-3282(97)00268-8
关键词:
摘要: Previous studies have shown that C3H/HeJ (C3H) mice higher peak bone density than C57BL/6J (B6) mice, at least in part because of differences rates resorption. The current were intended to examine the alternative, additional hypothesis greater C3H might also be a consequence increased formation. To end, we measured two presumptive, indirect indices formation and osteoblast number these inbred strains mice: alkaline phosphatase (ALP) activity serum, bones, cells; ALP-positive colony-forming units (CFU) marrow stromal cell cultures. We found had serum levels ALP B6 6 (118 vs. 100 U/L, p < 0.03) 32 weeks age (22.2 17.2 0.001). Tibiae from contained tibiae (417 254 mU/mg protein, 0.02) 14 (132 79 0.001), as did monolayer cultures bone-derived cells explants 7.5-week-old calvariae femora (8.2 times more, 0.02, 4.6 0.001, respectively). Monolayer prepared by collagenase digestion newborn 6-week-old showed similar strain-dependent ALP-specific (p 0.001 for each). Our more CFU 8-week-old compared with (72.3 26.1 CFU/culture dish, A result was seen production age, difference greatest numbers cells. Because skeletal is product osteoblasts has been correlate formation, believed reflect osteoprogenitor cells, data are consistent general may number. further suggest due combination decreased resorption
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