Inversion of lithium heparin gel tubes after centrifugation is a significant source of bias in clinical chemistry testing.

摘要: Abstract Background This study was planned to establish whether random orientation of gel tubes after centrifugation may impair sample quality. Materials and methods Eight were collected from 17 volunteers: 2 Becton Dickinson (BD) serum tubes, Terumo BD lithium heparin tubes. One patient's tube for each category kept in a vertical, closure-up position 90 min (“upright”), whereas paired underwent bottom-up inversion every 15 min, (“inverted”). Immediately this period time, 14 clinical chemistry analytes, indices complete blood count then assessed all Results Significant increases found phosphate lipaemic index inverted along with AST, calcium, cholesterol, LDH, potassium, hemolysis index, leukocytes, erythrocytes platelets limited The desirable quality specifications exceeded potassium Residual erythrocytes, cellular debris also significantly increased Conclusions Lithium should be maintained centrifugation.