Presence of simian virus 40 sequences in malignant mesotheliomas and mesothelial cell proliferations

摘要: Malignant mesotheliomas (MMs) are pleural-, pericardial-, or peritoneal-based neoplasms usually associated with asbestos exposure. Mesothelial cells biphasic and may give rise to epithelial sarcomatous MMs. In addition, benign atypical proliferations of mesothelial occur in response many stimuli. There have been recent reports simian virus 40 (SV40) DNA large T antigen (Tag) sequences pleural To further understand the relationship between SV40, MMs, proliferations, we studied 118 MMs from multiple sites Germany North America, including 93 pleural, 14 mixed 11 peritoneal 12 adjacent noninvasive tumor foci were identified separately. Information about exposure (detailed history and/or microscopic examination for bodies) was available 43 German patients. 13 examples reactive mesothelium 20 lung cancers United States tested. extracted frozen nontumorous tissues after microdissection archival formalin-fixed, paraffin-embedded microslides. Two rounds PCR performed primers SVFor 3 SVRev, which amplify a 105 bp region specific SV40 Tag. The specificity product confirmed some cases by sequencing. Our major findings were: 1) Specific viral present 57% invasive both origin. No significant geographic differences found, equally suitable analysis. 2) no apparent presence 3) surface (noninvasive) components 4) not detected histologies. 5) Viral two samples (15%) mesothelium. 6) Lung lacked sequences, as did non-malignant demonstrate origin their absence tumors component. be malignant cells, but they absent cancers.