Immunohistochemical Localization of Glutathione S-Transferases in Human Lung
作者： Harri Vainio , John D. Hayes , Sisko Anttila , Kirsti Husgafvel-Pursiainen , Ari Hirvonen
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摘要: Glutathione S-transferases (GST) detoxify a number of carcinogenic electrophiles including diol-epoxide metabolites polycyclic aromatic hydrocarbons. The distribution GSTs A1/A2, M1, M2, M3, and P1 has been studied in lung tissue from 32 subjects by immunohistochemistry using rabbit polyclonal antibodies. GSTA1/A2 GSTP1 were found to be the most abundant human lung, being present bronchial bronchiolar epithelium all individuals studied. staining intensity for varied more than that between individuals. GSTM1, polymorphic mu-class enzyme, was ambiguously detected and, if expressed, is at very low levels. GSTM2, striated muscle-specific isozyme, occurred minimally terminal airways, GSTM3, an enzyme broad extrahepatic occurrence, observable ciliated airway smooth muscle lung. GSTM3 minimal intense individuals; epithelium, it current smokers exsmokers. immunostaining general decreasing distal contrast previously described peripheral localization hydrocarbons activating P450IA1 enzyme. wall suggests GST polymorphisms may contribute susceptibility, especially tumors tobacco smokers.
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