Chronic Relapsing EAE: Its Application to Study of Human Inflammatory Demyelinating Diseases

摘要: In 1933, Rivers et al. [23] reported that they had observed an encephalomyelitis with myelin destruction in 2 of 8 monkeys received repeated IM injections aqueous emulsion and alcohol-ether extracts normal rabbit brain. Two years later, Schwentker [22] confirmed these original observations, stressing 7 developed demyelinating encephalomyelitis. The procedure used by al., followed confirming reports Ferraro Jervis [3], involved (46–85) emulsions This technique produced morphological lesions various ages sizes, gave a very low mortality rate. However, it was time consuming (3 inoculations week, up to 85 injections) difficult apply small animals. Since the goal experiments determine what component brain tissue causes encephalomyelitis, there great need for method which would shorten required induce disease. solved when Freund McDermott [5] demonstrated new emulsifying antigens adjuvants aquafor, paraffin oil, heat-killed tubercle bacilli. facilitated experimental production inception its symptoms one or two Freund’s adjuvant [15].