Expression and DNA methylation alterations of seven cancer-associated 3p genes and their predicted regulator miRNAs (miR-129-2, miR-9-1) in breast and ovarian cancers

摘要: The methylation of promoter CpG islands and interactions between microRNAs (miRNAs) messenger RNAs (mRNAs) target genes are considered two crucial epigenetic mechanisms for inducing gene pathway deregulation in tumors. Here, the expression levels seven cancer-associated 3p (RASSF1(isoform A), RARB(isoform 2), SEMA3B, RHOA, GPX1, NKIRAS1, CHL1) their predicted regulator miRNAs (miR-129-2, miR-9-1) were analyzed breast (BC, 40 samples) ovarian (OC, 14 cancers using RT-PCR qPCR. We first revealed a negative correlation level miR-129-2 precursor RASSF1(A) GPX1 mRNA BC (Spearman's coefficient (rs) was − 0.26 both cases). Similar results observed RASSF1(A), RARB(2), CHL1 OC (rs range 0.48 to 0.54). Using methylation-specific PCR, significant shown hypermethylation down-regulation MIR-129-2, MIR-9-1 = 0.41 0.75) 0.67). demonstrated high frequency MIR-129-2 SEMA3B (up 45 48%) (69 (41 samples). Moreover, we positive up-regulation 0.38 0.42, respectively). QPCR analysis mature additional sample set (24 them 0.41) that strengthened obtained during level. In summary, data indicate involvement studied coding miRNA suggest via direct interaction or/and mediators common pathways (according KEGG, Gene Ontology (FDR < 0.01), GeneCards data) examined gynecological