Lipidomics: practical aspects and applications.

摘要: Lipidomics is the characterization of molecular species lipids in biological samples. The polar that comprise bilayer matrix constituent cell membranes living tissues are highly complex and number many hundreds distinct lipid species. These differ nature group representing different classes lipid. Each class consists a range depending on length, position attachment unsaturated double bonds associated fatty acids. origin this complexity described biochemical processes responsible for homeostasis composition each morphologically-distinct membrane considered. practical steps have been developed isolation there from, their storage, separation, detection identification by liquid chromatography coupled to mass spectrometry described. Application lipidomic analyses examples where clinical screening lipidoses collaboration with facilities considered from user point view.