Cryopreservation-induced human sperm DNA damage is predominantly mediated by oxidative stress rather than apoptosis

作者: Laura K Thomson , Steven D Fleming , R John Aitken , Geoffry N De Iuliis , J-A Zieschang

DOI: 10.1093/HUMREP/DEP214

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摘要: results: Cryopreservation led to a significant increase in percentage DNA fragmentation, 8OHdG and caspase positive cells (P , 0.001). Percentage fragmentation was positively correlated with before (r ¼ 0.756, P 0.001) after cryopreservation 0.528, 0.017). The addition of 50 100 mM genistein the cryoprotectant had protective effect on sperm although inhibitor demonstrated no difference control. conclusions: Human is associated an oxidative stress during cryopreservation, rather than activation caspases apoptosis. estrogenic compound may be useful reducing this but larger trials are needed confirm this.

参考文章(59)
Rapone S, Dondero F, Pulcinelli Fm, Mazzilli F, Gazzaniga Pp, Rossi T, Sabatini L, Human sperm cryopreservation and reactive oxygen species (ROS) production. Acta Europaea fertilitatis. ,vol. 26, pp. 145- 148 ,(1995)
J P Twigg, P A Milne, E L Gordon, N Fulton, R J Aitken, D S Irvine, DNA Integrity in Human Spermatozoa: Relationships With Semen Quality Journal of Andrology. ,vol. 21, pp. 33- 44 ,(2000) , 10.1002/J.1939-4640.2000.TB03273.X
J Huang, M Nasr, Y Kim, H.R. Matthews, Genistein inhibits protein histidine kinase. Journal of Biological Chemistry. ,vol. 267, pp. 15511- 15515 ,(1992) , 10.1016/S0021-9258(19)49564-1
Jiankun Cui, Eric H. Holmes, Thomas G. Greene, Philip K. Liu, Oxidative DNA damage precedes DNA fragmentation after experimental stroke in rat brain The FASEB Journal. ,vol. 14, pp. 955- 967 ,(2000) , 10.1096/FASEBJ.14.7.955
H. Wei, R. Bowen, Q. Cai, S. Barnes, Y. Wang, Antioxidant and antipromotional effects of the soybean isoflavone genistein. Experimental Biology and Medicine. ,vol. 208, pp. 124- 130 ,(1995) , 10.3181/00379727-208-43844
Ai Wei Wang, Hua Zhang, Isao Ikemoto, Deborah J. Anderson, Kevin R. Loughlin, Reactive oxygen species generation by seminal cells during cryopreservation. Urology. ,vol. 49, pp. 921- 925 ,(1997) , 10.1016/S0090-4295(97)00070-8
Floris WPC van Overveld, Guido RMM Haenen, Johann Rhemrev, Jan PW Vermeiden, Aalt Bast, None, Tyrosine as important contributor to the antioxidant capacity of seminal plasma Chemico-Biological Interactions. ,vol. 127, pp. 151- 161 ,(2000) , 10.1016/S0009-2797(00)00179-4
Soliman I. Peris, Jean-François Bilodeau, Maurice Dufour, Janice L. Bailey, Impact of cryopreservation and reactive oxygen species on DNA integrity, lipid peroxidation, and functional parameters in ram sperm. Molecular Reproduction and Development. ,vol. 74, pp. 878- 892 ,(2007) , 10.1002/MRD.20686