Protein oxidative damage and heme oxygenase in sunlight-exposed human skin: roles of MAPK responses to oxidative stress.

摘要: Oxidative stress derived from ultraviolet (UV) light in sunlight induces different hazardous effects the skin, including sunburn, photo-aging and DNA mutagenesis. In this study, protein-bound lipid peroxidation products 4-hydroxy-2-nonenal (HNE) oxidative damage marker 8-hydroxy-2'-deoxyguanosine (8OHdG) were investigated chronically sun-exposed sun-protected human skins using immunohistochemistry. The levels of antioxidative enzymes, such as heme oxygenase 1 2, Cu/Zn-SOD, Mn-SOD catalase, also examined. is implicated activation signal transduction pathways, mitogen-activated protein kinase (MAPK). Therefore, expression distribution phosphorylated p38 MAPK, Jun N-terminal (JNK) extracellular signal-regulated (ERK) observed. Skin specimens obtained surgical margins. Chronically sunlight-exposed skin samples taken ante-auricular (n = 10) sunlight-protected post-auricular 10). HNE was increased but not skin. oxygenase-2 markedly compared with contrast, intensity immunostaining catalase between two areas. Phosphorylated MAPK JNK accumulated dermis epidermis, respectively. These data show that particular anti-oxidative enzymes function protective factors Taken together, our results suggest (1) (2) may be responsible for stress.