The effect of weightbearing and limb load cycling on equine lamellar perfusion and energy metabolism measured using tissue microdialysis.

摘要: Reasons for performing study: Lamellar perfusion is thought to be affected by weightbearing and limb load cycling; this may critical in the development of supporting laminitis. Objectives: To document effects unilateral altered cycling on lamellar energy metabolism perfusion. Study design: Randomised, controlled (within subject), experimental trial. Methods: Nine Standardbred horses were instrumented with microdialysis probes foot tissue skin (over tail base). Urea (20 mmol/l) was added perfusate. Samples collected every 15 min a 1 h control period, then during periods (opposite held off ground h); enhanced static (instrumented lifted 10 s 30 min); reduced activity (i.v. detomidine sedation) continuous walking (30 min). Dialysate concentrations glucose, lactate, pyruvate urea measured lactate:glucose (L:G) lactate:pyruvate (L:P) ratios calculated. For each intervention, values compared baseline using nonparametric statistical testing. Results: dialysate glucose increased L:G decreased significantly cycling. Glucose increased, L:G, L:P walking. Simultaneous did not change significantly. There no significant changes or after administration, but only latter resulted decrease frequency. Conclusions: Increases frequency (particularly walking) caused consistent Unilateral (1 h) sedation-induced reduction have detectable effect More research needed confirm role hypoperfusion laminitis, strategies increase important prevention.