Polysaccharide fractions of Caesalpinia ferrea pods: potential anti-inflammatory usage.

摘要: Abstract Ethnopharmacological relevance Caesalpinia ferrea (Caesalpinioideae), known as pau-ferro or juca, has been used in the traditional medicine North and Northeast of Brazil inflammatory disorder, among others. Thus, experimental evaluation anti-inflammatory activity extracts fraction polysaccharides pods, correlation with its popular use is important. Materials methods Total (TPL) were applied to ion exchange chromatography eluted stepwise. Paw edema was induced s.c. by λ-carrageenan, dextran, histamine, serotonin, compound 48/80, bradykinin, prostaglandin E 2 (PGE ) l -arginine analyzed plethysmometry protein leakage spectrophotometry. Peritonitis i.p. carrageenan N-formyl-methionyl-leucyl-phenylalanine (fMLP) 4 h later for leukocyte migration leakage. Animals treated i.v. TPL polysaccharide fractions (0.01, 0.1, 1 mg/kg) 30 min before stimuli both models. Toxicity (variation body/organ mass hematological/biochemical parameters) evaluated after seven-day treatment most active (1 mg/kg; i.v.). Results Chromatography (2.8% yield) provided three major (FI, FII, FIII). At 1 mg/kg, inhibited paw (60%) FIII (fraction presenting high carbohydrate low content) parameters following stimuli: (70%), dextran (53%), histamine (65%), serotonin (62%), bradykinin (60%), PGE (63%), nitric oxide (61%) 48/80 (36%). Additionally, at 1 mg/kg carrageenan-induced animals intact mast cells, but only late phase those degranulated cells elicited 48/80. Moreover, cell model peritonitis (88%) fMLP (64%), being well tolerated animals. Conclusions Extracts pods exhibit potent via negative modulation NO released edema, showing involvement cells. could be interfering not vascular, also cellular events, revealing an important component traditionally prepared remedies treat states.