BRAF Mutations in Metastatic Melanoma: A Possible Association with Clinical Outcome

摘要: Purpose: The RAS-RAF-mitogen-activated protein kinase pathways mediate the cellular response to growth signals. In melanocytes, BRAF is involved in cAMP-dependent Recently, activating mutations gene, were reported a large proportion of melanomas. We have studied gene and their association with clinical parameters. Experimental Design: analyzed exons 1, 11, 15 1 2 N-ras for 38 metastatic melanomas by PCR-single-strand conformation polymorphism direct sequencing. Kaplan-Meier survival multivariate analyses used correlate various Results: Mutations exon detected 26 (68%) 25 cases, mutation “hot spot” codon 600 gene. Three without carried amino acid substituting base changes at 61 proportional hazard (Cox) model, mutation, along stage melanomas, showed statistically significant ratio 2.16 (95% confidence interval 1.02–4.59; χ model 6.94, degrees freedom 2, P = 0.03) diminished duration treatment. cases longer disease-free (median 12 months) than 5 months), although this was not (Log-rank test 0.13). Conclusions: Our results, besides confirming high frequency also underline potential importance these disease outcome.