Evaluation of the effects of a new formulation of Leishmania major antigen in Balb/C and conventional white laboratory mice.
作者： Afshineh Latifynia , Saleh Mohaghegh Hazrati
DOI: 10.1016/S1684-1182(10)60022-9
关键词:
摘要: METHODS: A new antigen formulation was evaluated in type I and II mice. Leishmania major promastigotes cultured harvested at different growth stages, a cocktail made from the organisms. The preparations were tested for sterility contamination by endotoxin. Five methods utilized to produce crude preparation. protein levels of measured using Lowry method, antigens intradermally injected protocols After 38 days, all mice euthanized with diethyl ether, spleens removed. Histological sections prepared, stained hematoxylin eosin changes splenic white pulp (SWP) studied microscopically. RESULTS: Compared control groups, there drastic change structure. size increases SWP dependent on injection group mouse strain. There remarkable expansion lymphoid follicles treated groups both strains. CONCLUSION: able stimulate expand constituents spleen tissue. is where immune responses antibodies are produced. Therefore,
cabdirect.org
elsevier.com
sci-hub.se 参考文章(34)
D M Russo, E M Carvalho, J M Burns, R J Armitage, S G Reed, W R McMaster, L L Button, K H Grabstein, Human T cell responses to gp63, a surface antigen of Leishmania. Journal of Immunology. ,vol. 147, pp. 3575- 3580 ,(1991)
Michel. DuBois, K. A. Gilles, J. K. Hamilton, P. A. Rebers, Fred. Smith, Colorimetric Method for Determination of Sugars and Related Substances Analytical Chemistry. ,vol. 28, pp. 350- 356 ,(1956) , 10.1021/AC60111A017
Ronald W Ellis, Development of combination vaccines Vaccine. ,vol. 17, pp. 1635- 1642 ,(1999) , 10.1016/S0264-410X(98)00424-1
Anthony Muyombwe, Martin Olivier, Marc Ouellette, Barbara Papadopoulou, Selective Killing ofLeishmaniaAmastigotes Expressing a Thymidine Kinase Suicide Gene Experimental Parasitology. ,vol. 85, pp. 35- 42 ,(1997) , 10.1006/EXPR.1996.4115
J. Convit, M. Ulrich, O. Zerpa, R. Borges, N. Aranzazu, M. Valera, H. Villarroel, Z. Zapata, I. Tomedes, Immunotherapy of American cutaneous leishmaniasis in Venezuela during the period 1990–1999 Transactions of The Royal Society of Tropical Medicine and Hygiene. ,vol. 97, pp. 469- 472 ,(2003) , 10.1016/S0035-9203(03)90093-9
J.O. Olobo, C.O. Anjili, M.M. Gicheru, P.A. Mbati, T.M. Kariuki, J.I. Githure, D.K. Koech, W.R. McMaster, Vaccination of vervet monkeys against cutaneous leishmaniosis using recombinant Leishmania ‘major surface glycoprotein’ (gp63) Veterinary Parasitology. ,vol. 60, pp. 199- 212 ,(1995) , 10.1016/0304-4017(95)00788-6
Farrokh Modabber, Experiences with vaccines against cutaneous leishmaniasis: of men and mice. Parasitology. ,vol. 98, pp. 49- 60 ,(1989) , 10.1017/S0031182000072243
C. L. JAFFE, R. SHOR, H. TRAU, J. H. PASSWELL, Parasite antigens recognized by patients with cutaneous leishmaniasis Clinical and Experimental Immunology. ,vol. 80, pp. 77- 82 ,(2008) , 10.1111/J.1365-2249.1990.TB06444.X
S. C. F. MENDONÇA, D. G. RUSSELL, S. G. COUTINHO, Analysis of the human T cell responsiveness to purified antigens of Leishmania: lipophosphoglycan (LPG) and glycoprotein 63 (gp 63). Clinical and Experimental Immunology. ,vol. 83, pp. 472- 478 ,(2008) , 10.1111/J.1365-2249.1991.TB05663.X
Emanuela Handman, Linda L. Button, Robert W. McMaster, Leishmania major: Production of recombinant gp63, its antigenicity and immunogenicity in mice Experimental Parasitology. ,vol. 70, pp. 427- 435 ,(1990) , 10.1016/0014-4894(90)90127-X