Neoplastic Cells are a Rare Component in Human Glioblastoma Microvasculature

摘要: Microvascular proliferation is a key biological and diagnostic hallmark of human glioblastoma, one the most aggressive forms cancer. It has recently been suggested that stem-like glioblastoma cells have capacity to differentiate into functional endothelial cells, significant proportion vascular lining in tumors neoplastic origin. In principle, this finding could significantly impact efficacy development antiangiogenic therapies targeting vasculature. While potential cancer form endothelium culture seems clear, our clinical experience using variety molecular markers, do not contribute endothelial-lined vasculature primary glioblastoma. We sought confirm impression by analyzing vessels previously examined chromogenic situ hybridization (CISH) for EGFR immunohistochemistry mutant IDH1. Vessels containing expressing these definitive markers were identified small fraction tumors, but only 10% vessel profiles contained such when comprised less than cellularity cross section. Interestingly, rare intravascular showing amplification CISH or IDH1 protein located middle outer portions walls, amongst morphologic boundaries lining. To more directly address endothelium, we performed double labeling (Immunofluorescence/FISH) marker CD34 gene locus. Although positive unassociated with (<1%), analysis did identify amplified within linings, further supports observations incorporation tumor at best extremely rare, therefore questionable therapeutic significance.