Characterization of R132H mutation-specific IDH1 antibody binding in brain tumors.

摘要: Heterozygous point mutations of isocitrate dehydrogenase (IDH)1 codon 132 are frequent in grade II and III gliomas. Recently, we reported an antibody specific for the IDH1R132H mutation. Here investigate capability this to differentiate wild type mutated IDH1 protein central nervous system (CNS) tumors by Western blot immunohistochemistry. Results analysis correlated sequencing data. In blot, anti-IDH1R132H mouse monoclonal mIDH1R132H detected a band only tumors. Immunohistochemistry 345 primary brain demonstrated strong cytoplasmic weaker nuclear staining 122 cases. Correlation with direct 186 cases resulted consensus 177 Genetic retesting conflicting findings match 186/186 cases, all discrepancies resolving favor Intriguing is ability detect single infiltrating tumor cells. The very high frequency distribution mutation among entities allow highly sensitive discrimination various immunohistochemistry, such as anaplastic astrocytoma from glioblastoma or diffuse World Health Organization (WHO) pilocytic ependymoma. Noteworthy edge reactive gliosis.