Enhanced Survival During Murine Gram-negative Bacterial Sepsis by Use of a Murine Monoclonal Antibody

摘要: • We developed a murine monoclonal antibody (5B10 MAb) that reacted in vitro specifically to lipopolysaccharide (LPS) obtained from Escherichia coli 0111:B4. Enzyme-linked immunosorbent assay (ELISA) titers variety of gram-negative bacterial whole cell and LPS antigens demonstrated this may react with the O antigen portion 0111:B4 LPS. then examined ability protect mice vivo against challenge either viable bacteria or purified One milligram 5B10 MAb was administered intraperitoneally (IP) protected lethal E LPS, but no other type challenge. Protection occurred an dose-dependent manner, as little 0.01 mg enhanced survival. concluded IP pretreatment single would sepsis endotoxemia animal model anti-LPS specificity sufficient condition for during bacteremia, confirming importance pathogenesis sepsis. ( Arch Surg 1985;120:50-53)