Regional and cellular distribution of neural visinin-like protein immunoreactivities (VILIP-1 and VILIP-3) in human brain

摘要: Neural visinin-like proteins (VILIPs) are members of the neuronal subfamily intracellular EF-hand calcium sensor termed NCS family, which thought to play important roles in cellular signal transduction. While numerous studies suggest a wide but uneven distribution these rat and chicken brain, their location in, possible significance for, human remains be established. We used specific polyclonal antisera map brain for VILIP-1 VILIP-3 immunoreactivities. was detected cortical pyramidal cells interneurons, septal, subthalamic hippocampal neurons (subfields CA1 CA4 especially hilar interneurons) as well cerebellar Golgi, basket, granule, stellate dentate nucleus neurons. Purkinje were free immunoreaction. more restricted its distribution. It identified subpopulation granule Further, belonging different nuclei stem multiple subcortical nerve stained protein 3. A weak immunoreaction appeared Intracellularly immunoreactivity perikarya, dendrites some axons. Sometimes, immunostaining found neuropil. Glia did not express proteins. Our findings support, from neuroanatomical viewpoint, idea that may relevance signalling CNS.