Detection and discrimination of four Aspergillus section Nigri species by PCR

摘要: Species of Aspergillus section Nigri are not easily distinguished by traditional morphological techniques, and typically identified DNA sequencing methods. We developed four PCR primers to distinguish between niger, welwitschiae, carbonarius tubingensis, based on species-conserved differences in the calmodulin gene sequence. amplification from total using these was species specific; no occurred nontarget for each primer pair. Species-specific could mixed templates, indicating a utility determining culture uniformity isolated strains. In addition, with sets, be detected soil following mixed-species inoculation spores. This indicates that species-specific may useful distribution environmental samples without need identification strains, as well detecting infrequently culture-based methods. Significance Impact Study Aspergillus includes several produce ochratoxin A fumonisin, two mycotoxins impacting food safety human health. However, is difficult sequencing. Using sequences, we designed among Aspergillus niger, tubingensis accurately more quickly than sequence analysis. demonstrated methods can used detect identify within populations fungal isolation. will facilitate assessing at-risk foods potential mycotoxin contamination species.