W14/15 esterase gene haplotype can be a genetic landmark of cultivars and species of the genus Gentiana L.

摘要: We have identified multiple alleles for a single gene termed W14/15. This encodes closely related but not identical proteins W14 and W15 that accumulate in overwinter buds of Gentiana triflora (Takahashi et al. Breed Sci 56:39-46, 2006; Hikage Mol Genet Genomics 278:95-104, 2007). In this study, structural analysis the W14/15 was carried out 21 different gentian lines/cultivars consisting 5 species, to survey species- or line/cultivar-specific haplotypes. Within samples examined, variant forms were found. Those categorized into seven major types (type I-VII) ten subtypes based on presence three short insertion/deletion sites, RFLP several SNP sites. Each line/cultivar had distinct set variants an allelic pair. Phylogenetic showed cluster groups are characteristic i.e., G. triflora, scabra, pneumonanthe, septemfida unknown species other than former four. addition, within same sets haplotypes Thus, provide useful landmarks resolve phylogenies genus section Gentiana, as well analyze pedigree breeding history cultivars derived from those sp.