Methods for in vitro expansion of hematopoietic stem cells

作者: Donald Lavelle , Joseph De Simone , Yogen Saunthararajah , Mohammed Milhem , Ronald Hoffman

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摘要: The invention relates to methods of obtaining compositions for generating multipotent hematopoietic stem progenitor cells comprising expansion hematopietic in the presence HDACI and IDM. Methods enriched megakaryocyte are also provided. Compositions populations obtained therefrom provided by invention.

参考文章(90)
M M Sanchez del Pino, G Lopez-Rodas, R Sendra, V Tordera, Properties of the yeast nuclear histone deacetylase Biochemical Journal. ,vol. 303, pp. 723- 729 ,(1994) , 10.1042/BJ3030723
R. E. Ploemacher, E. A. W. Blokland, S. Neben, D. A. Breems, Frequency analysis of human primitive haematopoietic stem cell subsets using a cobblestone area forming cell assay. Leukemia. ,vol. 8, pp. 1095- 1104 ,(1994)
Joel Yisraeli, Moshe Szyf, Gene Methylation Patterns and Expression Springer, New York, NY. pp. 353- 378 ,(1984) , 10.1007/978-1-4613-8519-6_16
Steven Kessler, Douglas H. Robinson, Thomas A. Davis, In vitro amplification/expansion of CD34+ stem and progenitor cells ,(1993)
John E. Brandt, Amelia M. Bartholomew, Jeffrey D. Fortman, Mary C. Nelson, Edward Bruno, Luci M. Chen, Julius V. Turian, Thomas A. Davis, John P. Chute, Ronald Hoffman, Ex vivo expansion of autologous bone marrow CD34(+) cells with porcine microvascular endothelial cells results in a graft capable of rescuing lethally irradiated baboons. Blood. ,vol. 94, pp. 106- 113 ,(1999) , 10.1182/BLOOD.V94.1.106.413K01_106_113
Curt I. Civin, Human stem cells ,(1984)