Functional allelic loss detected at the protein level in archival human tumours using allele-specific E-cadherin monoclonal antibodies.

摘要: Immunohistochemical analysis has been used to show that expression of the homophilic cell-to-cell adhesion molecule, E-cadherin, is frequently altered in human cancers, including gastric and breast carcinoma. Besides genetic down-regulation, structural mutations such as in-frame deletions exon 8 9 were found; these may affect binding monoclonal antibodies for immunohistochemical analysis. In this study it was found HECD-1 E9, two often E-cadherin immunoanalysis, react with epitopes present at least part 9, respectively. This generated characterized a mutation-specific antibody, E-cad delta 8-1, reacting mutant protein lacking but not wild-type molecule. By using 8-1 HECD-1, possible separately analyse immunoreactivity normal proteins, respectively, an allele-specific manner archival material. A similar performed E9 previously antibody 9-1. Typically, cancer harbouring splice site gene mutations, proteins expressed detected malignant tissues. These results indicate variant-specific can be identify differentially proteins. For different should exclude alterations resulting failure detect protein.