New enzyme-linked immunosorbent assay for glycocalicin in plasma.

摘要: A new sandwich-type enzyme-linked immunosorbent assay for quantifying glycocalicin, a proteolytic fragment of platelet membrane glycoprotein Ib, is described. The based on the use two monoclonal antibodies raised against Ib and involves avidin-biotin technique. detection limit 7 micrograms/L range glycocalicin determined in plasma 0.01 to 1 mg/L. Assay time 2 h. intra-assay CV ranged from 3.6% 5.2%, interassay 5.4% 8.0%. Analytical recovery purified added pool averaged 96%. In 36 healthy subjects, mean concentration was 0.36 (SD 0.07) mg/L (2.7 nmol/L). We conclude that this suitable measuring also more sensitive precise than previously published immunoassays competitive binding assay.