Ultraviolet B irradiation increases keratin 5 and keratin 14 expression through epidermal growth factor receptor of SV40-transformed human keratinocytes
作者： Motoshi Kinouchi , Hidetoshi Takahashi , Yasuhiro Itoh , Akemi Ishida-Yamamoto , Hajime Iizuka
DOI: 10.1007/S00403-001-0284-9
关键词:
摘要: Keratin intermediate filaments are heteropolymers composed of type I and II keratins. Ultraviolet B (UVB) irradiation induces keratin expression by keratinocytes. Using SV40-transformed human keratinocytes (SVHK), we investigated the effect UVB on expression. (10 mJ/cm2) increased 5 14 mRNAs proteins without affecting cell viability. Upregulation was dependent dose radiation: observed at mJ/cm2 maximal 10 mJ/cm2. Higher doses (more than were cytotoxic. Expression 1 marginal in SVHK not affected either mRNA or protein level UVB. The stimulatory effects also cultured normal (NHK) HaCaT tyrosine kinase inhibitor, genistein, epidermal growth factor (EGF) receptor AG1429, significantly suppressed increase SVHK. In contrast, suppressive with C H-7. Furthermore, pretreatment neutralizing anti-EGF antibody UVB-induced SVHK, NHK cells. did affect steady-state TGF-α Immunoprecipitation immunohistochemical studies revealed that induced EGF activation absence TGF-α. These results indicate increases through direct
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