Fas ligand is present in tumors of the Ewing's sarcoma family and is cleaved into a soluble form by a metalloproteinase.

摘要: Fas ligand (FasL) exists in transmembrane and soluble forms induces apoptosis on cross-linking with the receptor. We evaluated biological significance of FasL 61 tumor tissues 9 cell lines Ewing's sarcoma family tumors (ESFT). was present 62.5% 79.4% primary ESFT. Metastatic had higher expression (95%), suggesting association a metastatic phenotype. detected cytoplasm membrane ESFT cells by immunofluorescence. Western blotting revealed cytosolic extracts conditioned media. Both induced Fas-sensitive Jurkat co-culture experiments or their Treatment phenanthroline synthetic metalloproteinase inhibitor BB-3103 reduced levels media, that ESFT, is processed released extracellular milieu. The may serve to attack immune system and/or interfere binding Fas, results down-regulation FasL. Synthetic inhibitors modify ratio