Malaria-Cutaneous Leishmaniasis Co-infection: Influence on Disease Outcomes and Immune Response.

摘要: Malaria and Cutaneous Leishmaniasis are co-endemic throughout large regions in tropical countries co-infection may impact the evolution of host-parasite interactions. In present study, we evaluate Malaria/Leishmaniasis disease outcome, Th1/Th2 cytokine levels CD4 CD8 T-cell profiles a murine model (BALB/c) P. yoelii 17XNL (Py) L. amazonensis (La) or braziliensis (Lb). parasitaemia was assessed through blood strains stained with Giemsa. Leishmania lesions were monitored digital caliper parasite loads determined by limiting-dilution assay. Serum IFN-γ, TNF, IL-2, IL-4, IL-6, IL-10, IL-17 using multiplexed bead assay expression CD3, T-cells markers Flow Cytometry thymus, spleens lymph nodes. Parasitaemia Lb+Py co-infected group lower than Py single-infected group, suggesting protective effect Lb progression. contrast, La+Py increased parasitaemia, patent infection induced mortality non-lethal infection. Regarding Leishmaniasis, presented smaller less ulceration animals. only transitory delay on development when compared to La mice. Decreased IFN-, IL-6 IL-10 observed serum groups, demonstrating modulation immune response co-infections. We an intense thymic atrophy which recovered earlier The T cell nodes did not differ between single except for decrease CD4+CD8+ cells also faster Our results suggest that change outcome. Interestingly outcome can be altered according specie involved. Alternatively reduced severity delayed onset leishmanial lesions. These alterations seem closely related changes as demonstrated alteration thymus/spleens phenotypes dynamics during