Response of osteoblasts and preosteoblasts to calcium deficient and Si substituted hydroxyapatites treated at different temperatures.

摘要: tHydroxyapatite (HA) is a calcium phosphate bioceramic widely used for bone grafting and augmenta-tion purposes. The biological response of HA can be improved through chemical microstructuralmodifications, as well by manufacturing it macroporous implants. In the present study, calciumdeficient hydroxyapatite (CDHA) Si substituted (SiHA) scaffolds havebeen prepared robocasting. order to obtain different microstructural properties, treated at 700◦C 1250◦C. have been characterized tested supports forboth osteoblast growth pre-osteoblast differentiation, fundamental requisite their potentialuse in tissue engineering. Morphology, viability, adhesion, proliferation, cell cycle, apoptosis, intra-cellular content reactive oxygen species interleukin-6 production were evaluated after contactof osteoblasts-like cells with CDHA SiHA materials. An adequate interaction cellsand preosteoblasts-like all these was observed. However, higher pro-liferation differentiation on 1250◦C lower adsorptionof albumin fibrinogen materials comparison those 700◦C, suggest bettertissue high temperature.