Production of Tissue Microarrays, Immunohistochemistry Staining and Digitalization Within the Human Protein Atlas

摘要: The tissue microarray (TMA) technology provides the means for high-throughput analysis of multiple tissues and cells. technique is used within Human Protein Atlas project global protein expression patterns in normal human tissues, cancer cell lines. Here we present assembly 1 mm cores, retrieved from microscopically selected representative into a single recipient TMA block. number size cores block can be varied approximately forty 2 to hundreds 0.6 cores. advantage using that large amount data rapidly obtained immunostaining protocol avoid experimental variability. Importantly, only limited scarce needed, which allows patient cohorts . Approximately 250 consecutive sections (4 μm thick) cut immunohistochemical staining determine specific different antibodies. In project, antibodies are generated towards all proteins acquire corresponding profiles both 144 individuals 216 patients, representing 20 most common forms cancer. Immunohistochemically stained on glass slides scanned create high-resolution images pathologists interpret annotate outcome immunohistochemistry. Images together with pathology-based annotation made publically available research community through portal (www.proteinatlas.org) (Figure 1) 3 4 map showing distribution relative abundance body. current version contains over 11 million 12.238 unique proteins, more than 61% encoded by genome.