Endogenous androgen receptor proteomic profiling reveals genomic subcomplex involved in prostate tumorigenesis

摘要: Androgen receptor (AR) is a key player in prostate cancer development and progression. Here we applied immunoprecipitation mass spectrometry of endogenous AR LNCaP cells to identify components the transcriptional complex. In total, 66 known novel interactors were identified presence synthetic androgen, most which critical for AR-driven cell proliferation. A subset required proliferation profiled using chromatin assays followed by sequencing, identifying distinct genomic subcomplexes interaction partners. Interestingly, three major subgroups identified, where selective gain function action tumorigenesis was found, dictated FOXA1 HOXB13. summary, combining proteomic approaches reveal subclasses complexes, differentiating normal behavior from oncogenic state. this process, expression has roles reprogramming cistrome interactome location-specific manner.