A Modified ELISA and Western Blot Accurately Determine Anti-Human Immunodeficiency Virus Type 1 Antibodies in Oral Fluids Obtained with a Special Collecting Device
作者： W. W. Emmons , S. F. Paparello , C. F. Decker , J. M. Sheffield , F. H. Lowe-Bey
DOI: 10.1093/INFDIS/171.6.1406
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摘要: Serum and saliva from 195 known human immunodeficiency virus (HIV)-seropositive patients 198 military personnel undergoing annual HIV serologic testing were evaluated in a prospective, blinded fashion for anti-HIV-1 antibodies. Oral specimens, collected with device designed to concentrate oral mucosal transudate whole saliva, tested by modified ELISA Western blot. was standard manner. All HIV-1-seropositive subjects had detectable anti-HIV- antibodies their ; 190 samples positive blot 5 indeterminate. None of the serum or fluid. The sensitivity, specificity, negative predictive values each 100%. appears be simple, safe, sensitive, specific method detecting
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