Expression of the flt3 receptor and its ligand on hematopoietic cells

摘要: Expression of the flt3 tyrosine kinase receptor and its ligand were examined on various murine human hematopoietic cell lines. Surface expression detected by flow cytometry using biotinylated or soluble Fc fusion protein (flt3R-Fc), respectively. Flt3 also Northern blot analysis. was expressed surface only two nine lines 15 lines, with positive cells representing B cell, early myeloid, monocytic lineages. Staining for revealed that seven screened cytometry. All assayed RNA analysis, but not all expressing mRNA had detectable expression. Cells T lineages at stages differentiation. Only OCI-AML-5, NALM-6, AML-193 coexpressed both ligand. The myeloid leukemic M1 terminally differentiate into macrophage-like under influence leukemia inhibitory factor (LIF). We found LIF-stimulated down-regulated levels receptor, up-regulated Although we could demonstrate functional in line, induce to differentiate.