A novel fixative for immunofluorescence staining of CD133-positive glioblastoma stem cells.
作者： Jonathan H. Sherman , Gerard T. Redpath , Jan A. Redick , Benjamin W. Purow , Edward R. Laws
DOI: 10.1016/J.JNEUMETH.2011.03.003
关键词:
摘要: Isolation of glioblastoma stem cells requires incubation tumor in a neural cell media. Neurospheres containing these are formed after approximately five-day period. These can then be analyzed for the presence markers. Immunofluorescence staining markers serve as valuable tool analyzing intact neurosphere directly Here we present use novel fixative (1,4-benzoquinone) immunoflourescence neurospheres.
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