An efficient surface modification using 2-methacryloyloxyethyl phosphorylcholine to control cell attachment via photochemical reaction in a microchannel

摘要: This report describes a direct approach for cell micropatterning in closed glass microchannel. To control the adhesiveness inside microchannel, application of an external stimulus such as ultraviolet (UV) was indispensible. technique focused on use modified 2-methacryloyloxyethyl phosphorylcholine (MPC) polymer, which is known to be non-biofouling compound that photocleavable linker (PL), localize cells via connection amino-terminated silanized surface. Using UV light illumination, MPC polymer selectively eliminated by photochemical reaction controlled attachment For suitable optimal illumination time and concentration suspension were investigated. After selective removal through photomask, MC-3T3 E1 vascular endothelial (ECs) localized only UV-exposed area. In addition, stability patterned ECs also confirmed culturing 2 weeks microchannel under flow conditions. Furthermore, we employed two different types same multiple polymer. Piccells both upper down streams respectively. When stimulated adenosine triphosphate (ATP), NO secreted from could detected fluorescence resonance energy transfer (FRET) Piccells, cell-based indicator. can powerful tool analyzing interaction research.