Reconstruction and Analysis of the lncRNA-miRNA-mRNA Network Based on Competitive Endogenous RNA Reveal Functional lncRNAs in Dilated Cardiomyopathy

摘要: Dilated cardiomyopathy (DCM) is an important cause of sudden death and heart failure with unknown etiology. Recent studies have suggested that long non-coding RNA (lncRNA) can interact microRNA (miRNA) indirectly mRNA through competitive endogenous (ceRNA) activities. However, the mechanism ceRNA in DCM remains unclear. In this study, a miRNA array was first performed using samples from patients healthy controls. For further validation, we conducted real-time quantitative reverse transcription (RT)-PCR doxorubicin-induced rodent model cardiomyopathy, revealing miR-144-3p miR-451a were down-regulated, miR-21-5p up-regulated. Based on theory, constructed global triple network data National Center for Biotechnology Information Gene Expression Omnibus (NCBI-GEO) our array. The lncRNA-miRNA-mRNA comprised 22 lncRNA nodes, 32 11 nodes. Hub nodes number relationship pairs then analyzed, results showed two lncRNAs (NONHSAT001691 NONHSAT006358) targeting miR-144/451 highly related to DCM. Then, cluster module random walk restart analyzed identified four (NONHSAT026953/NONHSAT006250/NONHSAT133928/NONHSAT041662) miR-21 significantly This study provides new strategy research or other diseases. Furthermore, lncRNA-miRNA may be regarded as candidate diagnostic biomarkers potential therapeutic targets