High-Throughput Flow Cytometry Screening Reveals a Role for Junctional Adhesion Molecule A as a Cancer Stem Cell Maintenance Factor
作者： Justin D Lathia , Meizhang Li , Maksim Sinyuk , Alvaro G Alvarado , William A Flavahan
DOI: 10.1016/J.CELREP.2013.11.043
关键词:
摘要: Stem cells reside in niches that regulate the balance between self-renewal and differentiation. The identity of a stem cell is linked with ability to interact its niche through adhesion mechanisms. To identify targets disrupt cancer stem cell (CSC) adhesion, we performed flow cytometry screen on patient-derived glioblastoma (GBM) identified junctional molecule A (JAM-A) as CSC mechanism essential for tumor growth. JAM-A was dispensable for normal neural stem/progenitor cell (NPC) function, expression was reduced brain versus GBM. Targeting compromised CSCs. negatively correlated to GBM patient prognosis. Our results demonstrate that GBM-targeting strategies can be screening receptors represents niche-driven maintenance.
researchgate.net 
core.ac.uk 
sci-hub.se 参考文章(61)
Rikke H Dahlrot, Jørn Herrstedt, Bjarne W Kristensen, Jacob Hjelmborg, Steinbjørn Hansen, A population-based study of high-grade gliomas and mutated isocitrate dehydrogenase 1 International Journal of Clinical and Experimental Pathology. ,vol. 6, pp. 31- 40 ,(2013)
Allan Spradling, Daniela Drummond-Barbosa, Toshie Kai, Stem cells find their niche Nature. ,vol. 414, pp. 98- 104 ,(2001) , 10.1038/35102160
Tejal U Naik, Meghna U Naik, Ulhas P Naik, Junctional adhesion molecules in angiogenesis Frontiers in Bioscience. ,vol. 13, pp. 258- ,(2008) , 10.2741/2676
Shauna H Yuan, Jody Martin, Jeanne Elia, Jessica Flippin, Rosanto I Paramban, Mike P Hefferan, Jason G Vidal, Yangling Mu, Rhiannon L Killian, Mason A Israel, Nil Emre, Silvia Marsala, Martin Marsala, Fred H Gage, Lawrence SB Goldstein, Christian T Carson, None, Cell-Surface Marker Signatures for the Isolation of Neural Stem Cells, Glia and Neurons Derived from Human Pluripotent Stem Cells PLoS ONE. ,vol. 6, pp. e17540- ,(2011) , 10.1371/JOURNAL.PONE.0017540
Tatyana N. Ignatova, Valery G. Kukekov, Eric D. Laywell, Oleg N. Suslov, Frank D. Vrionis, Dennis A. Steindler, Human cortical glial tumors contain neural stem-like cells expressing astroglial and neuronal markers in vitro. Glia. ,vol. 39, pp. 193- 206 ,(2002) , 10.1002/GLIA.10094
Mark Shackleton, François Vaillant, Kaylene J. Simpson, John Stingl, Gordon K. Smyth, Marie-Liesse Asselin-Labat, Li Wu, Geoffrey J. Lindeman, Jane E. Visvader, Generation of a functional mammary gland from a single stem cell Nature. ,vol. 439, pp. 84- 88 ,(2006) , 10.1038/NATURE04372
Eric A Severson, Charles A Parkos, Structural determinants of Junctional Adhesion Molecule A (JAM-A) function and mechanisms of intracellular signaling Current Opinion in Cell Biology. ,vol. 21, pp. 701- 707 ,(2009) , 10.1016/J.CEB.2009.06.005
Veronique Frattini, Vladimir Trifonov, Joseph Minhow Chan, Angelica Castano, Marie Lia, Francesco Abate, Stephen T Keir, Alan X Ji, Pietro Zoppoli, Francesco Niola, Carla Danussi, Igor Dolgalev, Paola Porrati, Serena Pellegatta, Adriana Heguy, Gaurav Gupta, David J Pisapia, Peter Canoll, Jeffrey N Bruce, Roger E McLendon, Hai Yan, Ken Aldape, Gaetano Finocchiaro, Tom Mikkelsen, Gilbert G Privé, Darell D Bigner, Anna Lasorella, Raul Rabadan, Antonio Iavarone, None, The integrated landscape of driver genomic alterations in glioblastoma Nature Genetics. ,vol. 45, pp. 1141- 1149 ,(2013) , 10.1038/NG.2734
Francesco Niola, Xudong Zhao, Devendra Singh, Angelica Castano, Ryan Sullivan, Mario Lauria, Hyung-song Nam, Yuan Zhuang, Robert Benezra, Diego Di Bernardo, Antonio Iavarone, Anna Lasorella, Id proteins synchronize stemness and anchorage to the niche of neural stem cells. Nature Cell Biology. ,vol. 14, pp. 477- 487 ,(2012) , 10.1038/NCB2490
Anita B Hjelmeland, Justin D Lathia, Sith Sathornsumetee, Jeremy N Rich, Twisted tango: brain tumor neurovascular interactions Nature Neuroscience. ,vol. 14, pp. 1375- 1381 ,(2011) , 10.1038/NN.2955