PDGF, TGF-β, and Heterotypic Cell–Cell Interactions Mediate Endothelial Cell–induced Recruitment of 10T1/2 Cells and Their Differentiation to a Smooth Muscle Fate

摘要: We aimed to determine if and how endothelial cells (EC) recruit precursors of smooth muscle pericytes induce their differentiation during vessel formation. Multipotent embryonic 10T1/2 were used as presumptive mural cell precursors. In an under-agarose coculture, EC induced migration via platelet-derived growth factor BB. in coculture with changed from polygonal spindle-shaped, reminiscent culture. Immunohistochemical Western blot analyses examine the expression (SM)-specific markers cultured absence presence EC. SM-myosin, SM22α, calponin proteins undetectable alone; however, all three SM-specific was significantly cocultured Treatment TGF-β phenotypic changes SM similar those seen cocultures. Neutralization cocultures blocked shape change. To assess ability contribute developing wall vivo, prelabeled grown a collagen matrix implanted subcutaneously into mice. The fluorescently marked became incorporated medial layer vessels where they expressed markers. These vitro vivo observations shed light on cell–cell interactions that occur development, well pathologies which developmental processes are recapitulated.