KRAS and BRAF mutation analysis can be reliably performed on aspirated cytological specimens of metastatic colorectal carcinoma.

摘要: N. K. B. Pang, M. E. Nga, S. Y. Chin, T.-M. Ismail, G. L. Lim, R. Soong and Salto-Tellez KRAS BRAF mutation analysis can be reliably performed on aspirated cytological specimens of metastatic colorectal carcinoma Background:  Sanger sequencing is one several reliable methods in use to detect mutations facilitate clinical patient selection for anti-epidermal growth factor receptor (EGFR) monoclonal antibody therapy unresectable adenocarcinoma (CRC). Most analyses are made pretreatment biopsy or resection specimens. There a scarcity published studies the suitability samples testing this setting. Methods:  DNA extraction was attempted 11 search-retrieved paired cases histological resections excisions CRC their corresponding (representing metastases) tested exon 2 3, as well 15 by sequencing. Only wild-type were subjected because setting with true diagnostic value, these mutually exclusive. Results:  Of analysed, only eight histology showed satisfactory quality Thus, cytology analysed. Seven same genotype both specimen carcinoma primary tumour (histological specimen), from which we derive an overall concordance rate 87.5%. The single discordant case likely difference it demonstrated again repeat samples. No detected four cases. Conclusion:  A range suitable testing, previously stained preparations cell blocks. These would highly valuable where material available testing.