Breast cancer models to study the expression of estrogen receptors with small animal PET imaging

摘要: Different animal models of estrogen positive tumors (ER+) were evaluated for their suitability to follow tumor response after various treatment protocols, using small positron emission tomography (PET). ER+ human breast cancer cell lines MCF-7 and T-47D, MDA-MB-231 as ER-; control, murine mammary ductal carcinomas MC4-L2, MC4-L3, MC7-L1, compared in vivo growth rate retention status. Tumor metabolic activity was estimated from the relative uptake (% injected dose/g) [18F]fluorodeoxyglucose (FDG) uptake, whereas ER content determined 16alpha-[18F]fluoroestradiol (FES) retention. F-18 values obtained by PET imaging confirmed tissue sampling radioactivity counting. Reliable measurements could be 200 microl or over. The grew at a slower failed accumulate FES; contrast, Balb/c MC7-L1 MC4-L2 well showed good both FDG FES. Chemotherapy hormone therapy delayed tumors, confirming an model therapeutic interventions. MC4-L3 also promising results but required presence progestative pellets grow. These data demonstrate that are suitable monitoring imaging.