Properties of cells derived from adenovirus-induced hamster tumors by long-term in vitro cultivation: I. Clonal stability of three biological characteristics

摘要: Abstract After several hundred generations in vitro, HT cells originally derived from type 12 adenovirus (Ad.12)-induced hamster tumors have retained their malignant character, the capacity to synthesize Ad.12-specific antigen(s), and a significantly reduced capacity, compared with that of normal cells, produce infectious 2 (Ad.2) response superinfection. Of four independently cell lines studied, one (HT2) consistently produces less Ad.2 than other three lines. No evidence has been obtained for “rescue” Ad.12 among yields Ad.2-superinfected cells. The low yield such is not due poor adsorption inoculum virus. Thirteen clones two (HT2 HT8) established after microdrop isolation single fact all tested characteristics suggests genetic homogeneity parental populations. In particular, differing extent which synthesis depressed reflected clones. argues against idea populations consist majority genetically stable “resistant” minority stably “susceptible”